Serotonin-storing secretory granules from thyroid parafollicular cells
نویسندگان
چکیده
منابع مشابه
Serotonin-storing secretory granules from thyroid parafollicular cells.
The subcellular storage of 5-HT was studied in sheep thyroid parafollicular cells. These cells are neural crest derivatives and were investigated as a serotonergic model system. Light and electron microscopic immunocytochemistry was used to examine the distributions of 5-HT, 45 and 56 kDa forms of 5-HT binding protein (SBP), and calcitonin. A single type of parafollicular cell was found that co...
متن کاملSeparation of Dissociated Thyroid Follicular and Parafollicular Cells : Association of Serotonin Binding Protein with Parafollicular Cells
Parafollicular cells (PC) of the sheep thyroid gland are neural crest derivatives that synthesize and release the biogenic amine serotonin (5-HT) as well as the hormone calcitonin . The thyroid also contains a highly specific serotonin-binding protein (SBP) . Separation of dissociated thyroid cells was done to study the cellular localization of SBP and to develop a means of isolating PC for stu...
متن کاملThyrotropin induces the acidification of the secretory granules of parafollicular cells by increasing the chloride conductance of the granular membrane
Secretory granules of sheep thyroid parafollicular cells contain serotonin, a serotonin-binding protein, and calcitonin. Parafollicular cells, isolated by affinity chromatography, were found to secrete serotonin when activated by thyrotropin (TSH) or elevated [Ca2+]e. TSH also induced a rise in [Ca2+]i. We studied the effect of these secretogogues on the pH difference (delta pH) across the memb...
متن کاملSeparation of dissociated thyroid follicular and parfollicular cells: association of serotonin binding protein with parafollicular cells
Parafollicular cells (PC) of the sheep thyroid gland are neural crest derivatives that synthesize and release the biogenic amine serotonin (5-HT) as well as the hormone calcitonin. The thyroid also contains a highly specific serotonin-binding protein (SBP). Separation of dissociated thyroid cells was done to study the cellular localization of SBP and to develop a means of isolating PC for study...
متن کاملA filamentous network surrounding secretory granules from mast cells.
A freezing-thawing method for rupturing mast cells has made it possible to show that each of the secretory granules is surrounded by a regular network of filaments forming a basket-like structure. The filaments attach to the membrane with hook-like structures. Immunoelectron microscopy of intact mast cells shows that actin occurs mainly in the subplasmalemmal space, whereas in ruptured cells it...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1987
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.07-12-04017.1987